Journal: Cell host & microbe

Article Title: IFI16 targets the transcription factor Sp1 to suppress HIV-1 transcription and latency reactivation

doi: 10.1016/j.chom.2019.05.002

Figure Lengend Snippet: (A-C) HEK293T cells were cotransfected with increasing doses of expression constructs for IFI16 (0.004, 0.02, 0.1, 0.5 and 2.5 μg) and constant quantities (2.5 μg) of the proviral HIV-1 NL4-3 construct. Cells and supernatants were harvested 40 hours post-transfection. Data show mean percentages (±SEM) relative to those detected in the absence of IFI16 (100%) and were derived from three experiments each performed in triplicate. * p < 0.05, ** p < 0.01, *** p < 0.001. (A) Infectious virus yield and p24 antigen levels in the HEK293T cell supernatants determined by TZM-b1 cell infection assay and p24 antigen ELISA, respectively. (B) Levels of different viral transcripts determined by qRT-PCR in multiplex reactions with GAPDH as control. (C) Expression of HIV-1 proteins, IFI16 and GAPDH in viral particles or cellular extracts was determined by Western blot.

Article Snippet: Rabbit anti-p24 antiserum obtained by immunization of rabbits , Eurogentec , N/A.

Techniques: Expressing, Construct, Transfection, Derivative Assay, Infection, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Multiplex Assay, Western Blot

Journal: Cell host & microbe

Article Title: IFI16 targets the transcription factor Sp1 to suppress HIV-1 transcription and latency reactivation

doi: 10.1016/j.chom.2019.05.002

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-p24 antiserum obtained by immunization of rabbits , Eurogentec , N/A.

Techniques: Recombinant, Purification, Transfection, Enzyme-linked Immunosorbent Assay, Cell Isolation, Mutagenesis, DNA Ligation, Luciferase, Cell Culture, Lysis, Magnetic Beads, Bicinchoninic Acid Protein Assay, Transcription Factor Assay, Activity Assay, Expressing, Construct, Plasmid Preparation, Binding Assay, Generated, Software